abstract：:Avoparcin was used as a feed additive in Norwegian broiler and turkey production from 1986 until 1995. It was banned due to the selection of VanA-type vancomycin-resistant enterococci (VRE) in animal husbandry and to reduce the potential for human exposure to VRE. The aim of the present study was to investigate the pr...

journal_title：Journal of applied microbiology

authors： Borgen K,Simonsen GS,Sundsfjord A,Wasteson Y,Olsvik O,Kruse H

更新日期：2000-09-01 00:00:00

abstract：:Twenty-nine Lactococcus lactis isolates from one traditional 24 h-old Pecorino Sardo cheese were characterized phenotypically, technologically and genotypically in order to assess the biodiversity within this wild microbial population. Two DNA-based techniques, plasmid profiling and PFGE, were used for the genetic typ...

journal_title：Journal of applied microbiology

authors： Mannu L,Paba A,Pes M,Scintu MF

更新日期：2000-08-01 00:00:00

abstract：:Pseudomonas fluorescens RO98, a raw milk isolate, was inoculated into McKellar's minimal salts medium and incubated at 25 degrees C for 48 h to allow production of protease. A zinc-metalloacid protease was purified from the cell-free concentrate by anion exchange and gel filtration chromatography. The purified proteas...

journal_title：Journal of applied microbiology

authors： Koka R,Weimer BC

更新日期：2000-08-01 00:00:00

abstract：:Cerein 7 is a peptidic antibiotic produced by Bacillus cereus Bc7 (CECT 5148) at the end of exponential growth but before sporulation onset. Cerein 7 has a broad spectrum of antibacterial activity against Gram-positive bacteria, but it is inactive against Gram-negative bacteria. The sequence of its amino-terminal end ...

journal_title：Journal of applied microbiology

authors： Oscáriz JC,Pisabarro AG

更新日期：2000-08-01 00:00:00

abstract：:Pathogens express virulence genes in response to the combination of environmental conditions present in the host environment. The crop is the first gastrointestinal environment encountered in birds. However, feed withdrawal alters the crop environment resulting in an increased pH, and decreased concentrations of lacta...

journal_title：Journal of applied microbiology

authors： Durant JA,Corrier DE,Stanker LH,Ricke SC

更新日期：2000-07-01 00:00:00

abstract：:The survival and environmental spread of Salmonella bacteria from domestic toilets was examined in homes, where a family member had recently suffered an attack of salmonellosis. In four out of six households tested, Salmonella bacteria persisted in the biofilm material found under the recess of the toilet bowl rim whi...

journal_title：Journal of applied microbiology

authors： Barker J,Bloomfield SF

更新日期：2000-07-01 00:00:00

abstract：:A total of 21 plant-associated Serratia plymuthica strains were characterized phenotypically by their nutritional patterns, susceptibility to antibiotics, antifungal and haemolytic properties, and genotypically by denaturing gradient gel electrophoresis (DGGE) of PCR-amplified 16S rDNA, PCR fingerprints using BOX prim...

journal_title：Journal of applied microbiology

authors： Berg G

更新日期：2000-06-01 00:00:00

abstract：:Klebsiella pneumoniae clinical isolates were selected according to the results of antibiotic susceptibility tests. Most of them were resistant to multiple antibiotics, including ampicillin, ceftazidime, cefotaxime and aminoglycosides. Large plasmids were observed in these Kl. pneumoniae strains by pulsed-field gel ele...

journal_title：Journal of applied microbiology

authors： Li L,Lim CK

更新日期：2000-06-01 00:00:00

abstract：:An alkalophilic, environmental micro-organism, Bacillus sp. BG-11, has been isolated and characterized. It produced 76 U ml-1 of chitinase in liquid batch fermentation after 72 h of incubation at 50 degrees C using chitin-enriched medium. The molecular weight of purified chitinase was estimated to be 41 kDa by SDS-PAG...

journal_title：Journal of applied microbiology

authors： Bhushan B

更新日期：2000-05-01 00:00:00

abstract：:The effects of steroidal saponins (SAP) isolated from Yucca schidigera extract on ruminal bacteria and fungi were investigated in pure culture studies. Prevotella bryantii, Ruminobacter amylophilus, Selenomonas ruminantium and Streptococcus bovis were cultured through ten 24-h transfers in ruminal fluid medium contain...

journal_title：Journal of applied microbiology

authors： Wang Y,McAllister TA,Yanke LJ,Cheeke PR

更新日期：2000-05-01 00:00:00

abstract：:D-values were obtained for Listeria monocytogenes and Yersinia enterocolitica at 50, 55 and 60 degrees C in vacuum-packed minced beef samples heated in a laboratory water-bath. The experiment was repeated using vacutainers, which allowed heating of the beef to the desired temperature before inoculation. D-values of be...

journal_title：Journal of applied microbiology

authors： Bolton DJ,McMahon CM,Doherty AM,Sheridan JJ,McDowell DA,Blair IS,Harrington D

更新日期：2000-04-01 00:00:00

abstract：:The regrowth of micro-organisms in the water distribution system is assisted by the presence of biodegradable organic matter (BDOC). The low concentration of available organic carbon in this type of water favours the growth of bacteria belonging to the Pseudomonadaceae family, as this group can grow better at low conc...

journal_title：Journal of applied microbiology

authors： Ribas F,Perramon J,Terradillos A,Frias J,Lucena F

更新日期：2000-04-01 00:00:00

abstract：:Rapid enumeration methods based on the enzymatic hydrolysis of 4-methylumbelliferyl-beta-D-galactoside and 4-methylumbelliferyl-beta-D-glucuronide were optimized for freshwaters. The enzymes beta-D-galactosidase (GALase) and beta-D-glucuronidase (GLUase) were shown to be already induced in freshwaters when tested, res...

journal_title：Journal of applied microbiology

authors： George I,Petit M,Servais P

更新日期：2000-03-01 00:00:00

abstract：:An extracellular alpha-amylase gene from the hyperthermophilic archaeon Pyrococcus woesei has been cloned and sequenced. The 1.4-kb protein-coding sequence is identical to that of the corresponding alpha-amylase gene of the closely related species P. furiosus. By using a shuttle cloning vector for halophilic bacteria,...

journal_title：Journal of applied microbiology

authors： Frillingos S,Linden A,Niehaus F,Vargas C,Nieto JJ,Ventosa A,Antranikian G,Drainas C

更新日期：2000-03-01 00:00:00

abstract：:The enzymatic activity and viability of Escherichia coli O157:H7 in natural river water was determined by flow cytometry. River water was collected at two sites (an agricultural area and an industrial area) on the Aigawa River (Osaka, Japan). To facilitate estimation of the physiology of E. coli O157 in natural river ...

journal_title：Journal of applied microbiology

authors： Tanaka Y,Yamaguchi N,Nasu M

更新日期：2000-02-01 00:00:00

abstract：:The volatile oils of black pepper [Piper nigrum L. (Piperaceae)], clove [Syzygium aromaticum (L.) Merr. & Perry (Myrtaceae)], geranium [Pelargonium graveolens L'Herit (Geraniaceae)], nutmeg [Myristica fragrans Houtt. (Myristicaceae), oregano [Origanum vulgare ssp. hirtum (Link) Letsw. (Lamiaceae)] and thyme [Thymus vu...

journal_title：Journal of applied microbiology

authors： Dorman HJ,Deans SG

更新日期：2000-02-01 00:00:00

abstract：:The effect of different levels of salt, sodium nitrite, polyphosphate and various sugars on growth, pigment production, protease activity and culture pH caused by Monascus purpureus was studied in broth medium and ground meat. The addition of sodium chloride (> 50.0 g l(-1)) and polyphosphate (> 3.0g l(-1)) to broth m...

journal_title：Journal of applied microbiology

authors： Tseng YY,Chen MT,Lin CF

更新日期：2000-01-01 00:00:00

abstract：:Water-buffalo Mozzarella (WBM) cheese is one of the several 'pasta filata' or stretched curd cheeses that originated in southern Italy, traditionally manufactured from raw milk employing natural whey starter cultures. Lactose- and galactose-fermenting yeasts isolated from WBM were studied to evaluate their role in the...

journal_title：Journal of applied microbiology

authors： Suzzi G,Lombardi A,Lanorte MT,Caruso M,Andrighetto C,Gardini F

更新日期：2000-01-01 00:00:00

abstract：:A number of bacterial strains used for meat fermentations were screened for proteolytic activity. A strain of Micrococcus which was found to be proteolytic was evaluated for the effects of environmental conditions on its proteolytic activity against pork myofibrillar proteins using response surface methodology. Three ...

journal_title：Journal of applied microbiology

authors： Kenneally PM,Fransen NG,Grau H,O'Neill EE,Arendt EK

更新日期：1999-12-01 00:00:00

abstract：:Cattle are considered the major reservoir for Escherichia coli O157:H7, one of the newly emerged foodborne human pathogens of animal origin and a leading cause of haemorrhagic colitis in humans. A sensitive test that can accurately and rapidly detect the organism in the food animal production environment is critically...

journal_title：Journal of applied microbiology

authors： Hu Y,Zhang Q,Meitzler JC

更新日期：1999-12-01 00:00:00

abstract：:The primary mode of transmission of the human pathogen Helicobacter pylori is unresolved. This study examined the possibility that H. pylori is water-borne. Because methods for the direct culture of H. pylori from water samples remain elusive, a microscopic technique was used for detection of this organism. Actively r...

journal_title：Journal of applied microbiology

authors： Hegarty JP,Dowd MT,Baker KH

更新日期：1999-11-01 00:00:00

abstract：:A number of phenotypic and molecular fingerprinting techniques, including physiological profiling (Biolog), restriction fragment length polymorphism (RFLP), enterobacterial repetitive intergenic consensus (ERIC) and a phage typing system, were evaluated for their ability to differentiate between 60 strains of Erwinia ...

journal_title：Journal of applied microbiology

authors： Toth IK,Bertheau Y,Hyman LJ,Laplaze L,López MM,McNicol J,Niepold F,Persson P,Salmond GP,Sletten A,van Der Wolf JM,Pérombelon MC

更新日期：1999-11-01 00:00:00

abstract：:Sorbitol fermenting bifidobacteria were evaluated as indicators of non-point source human faecal pollution to three sub-estuaries with elevated faecal coliform densities. Human-specific bifidobacteria correlated with identifiable human sanitary deficiencies in feeder streams to estuarine creeks in two of three watersh...

journal_title：Journal of applied microbiology

authors： Rhodes MW,Kator H

更新日期：1999-10-01 00:00:00

abstract：:The influence of growth phase on the evolution of cellular fatty acids (CFA) and survival of Sinorhizobium and Bradyrhizobium during desiccation and storage at different levels of relative humidity (R. H.) was studied. Lactobacillic, cis vaccenic and palmitic acids were the major fatty acids of S. meliloti RCR 2011, B...

journal_title：Journal of applied microbiology

authors： Boumahdi M,Mary P,Hornez J

更新日期：1999-10-01 00:00:00

abstract：:Two polychlorinated biphenyl-contaminated sites in the Czech Republic, a soil at Zamberk and a sediment sludge at Milevsko, were screened for the presence of chlorobenzoate degraders. Sixteen different chlorobenzoate degraders were isolated from the soil compared with only three strains isolated from the sediment. Fro...

journal_title：Journal of applied microbiology

authors： Pavlû L,Vosáhlová J,Klierová H,Prouza M,Demnerová K,Brenner V

更新日期：1999-09-01 00:00:00

abstract：:During the first half of the century, the majority of anthrax outbreaks in Canada occurred in the southern portions of Ontario and Quebec and were often associated with pastures contaminated by effluent from textile industries dealing with imported animal materials. In 1952, introduction of Federal regulations requiri...

journal_title：Journal of applied microbiology

authors： Dragon DC,Elkin BT,Nishi JS,Ellsworth TR

更新日期：1999-08-01 00:00:00

abstract：:Two abundant surface proteins, EA1 and Sap, are components of the Bacillus anthracis surface layer (S-layer). Their corresponding genes have been cloned, shown to be clustered on the chromosome and sequenced. EA1 and Sap each possess three 'S-layer homology' motifs. Single and double disrupted mutants were constructed...

journal_title：Journal of applied microbiology

authors： Fouet A,Mesnage S,Tosi-Couture E,Gounon P,Mock M

更新日期：1999-08-01 00:00:00

abstract：:The lethal toxin (LeTx) of Bacillus anthracis is the major virulence factor responsible for the death of infected animals and for cytolysis of cultured macrophages. Its catalytic component, LF, contains the characteristic zinc-binding motif of metalloproteases, it binds zinc and indirect evidence suggests that this hy...

journal_title：Journal of applied microbiology

authors： Vitale G,Pellizzari R,Recchi C,Napolitani G,Mock M,Montecucco C

更新日期：1999-08-01 00:00:00

abstract：:The achievements of a World Health Organization Anthrax Working Group, established in 1990, have been the production of two editions of guidelines on anthrax surveillance and control and the formulation of templates to assist countries in the construction of their surveillance and control programmes. The latter was ma...

journal_title：Journal of applied microbiology

authors： Turnbull PC,Hugh-Jones ME,Cosivi O

更新日期：1999-08-01 00:00:00

abstract：:Pseudomonas fluorescens DR54 showed antagonistic properties against plant pathogenic Pythium ultimum and Rhizoctonia solani both in vitro and in planta. Antifungal activity was extractable from spent growth media, and fractionation by semi-preparative HPLC resulted in isolation of an active compound, which was identif...

journal_title：Journal of applied microbiology

authors： Nielsen TH,Christophersen C,Anthoni U,Sørensen J

更新日期：1999-07-01 00:00:00

abstract：:The effect of electroporation (very short duration pulses of high voltage electricity) on the viability of Giardia cysts and Cryptosporidium oocysts, and on the viability of these organisms in the presence of free chlorine, combined chlorine, hydrogen peroxide and potassium permanganate, was examined. While electropor...

journal_title：Journal of applied microbiology

authors： Haas CN,Aturaliye D

更新日期：1999-06-01 00:00:00

abstract：:The changes in bacterial counts during the storage of a natural mineral water from a French spring were studied. Samples were taken from the spring and the bottling line. Viable cultivable (VC) bacteria were counted on R2A medium. Total counts, viable and dead bacteria were counted using the LIVE/DEAD Bac Light VIABIL...

journal_title：Journal of applied microbiology

authors： Defives C,Guyard S,Oularé MM,Mary P,Hornez JP

更新日期：1999-06-01 00:00:00

abstract：:In order to characterize the subtypes of Salmonella typhi which cause sporadic disease in Taiwan, 55 isolates of Salm. typhi obtained from unrelated patients of sporadic cases during 1992-96 were subjected to chromosomal DNA digestion and pulsed field gel electrophoresis (PFGE). When DNAs of these 55 Salm. typhi strai...

journal_title：Journal of applied microbiology

authors： Tsen HY,Lin JS,Hu HH,Liu PR,Wang TK

更新日期：1999-05-01 00:00:00

abstract：:The role of membrane fatty acid composition in the resistance of Pseudomonas aeruginosa ATCC 15442 to the bactericidal activity of didecyldimethyl ammonium bromide (DDAB) was investigated. In this study, the strain was sub-cultured in a medium with increasing DDAB concentrations. After adaptation, Ps. aeruginosa was a...

journal_title：Journal of applied microbiology

authors： Méchin L,Dubois-Brissonnet F,Heyd B,Leveau JY

更新日期：1999-05-01 00:00:00

abstract：:The classification of Bacillus thuringiensis strains has been revised and updated based on flagellar antigens which have been in use for many years. Sixty-nine serotypes and 13 sub-antigenic groups have now been identified, giving 82 serovars among the 3500 B. thuringiensis isolates of the IEBC Collection. The number ...

journal_title：Journal of applied microbiology

authors： Lecadet MM,Frachon E,Dumanoir VC,Ripouteau H,Hamon S,Laurent P,Thiéry I

更新日期：1999-04-01 00:00:00

abstract：:Energy dispersive analysis of X-rays (EDAX) was used to study the effects of chlorhexidine diacetate (CHA) and polyhexamethylene biguanide (PHMB) on Acanthamoeba castellanii. A high variation of elements occurred in untreated individual cells and only two elements, Cl (a biocide marker) and P, were investigated. X-ray...

journal_title：Journal of applied microbiology

authors： Khunkitti W,Hann AC,Lloyd D,Furr JR,Russell AD

更新日期：1999-03-01 00:00:00

abstract：:Application of high hydrostatic pressure (200, 300, 350 and 400 MPa) at 5 degrees C for 30 min to different micro-organisms, including Gram-positive and Gram-negative bacteria, moulds and yeasts, proved to be more effective in inactivating these organisms than treatments at 20 degrees C for 10 min and at 10 degrees C ...

journal_title：Journal of applied microbiology

authors： Arroyo G,Sanz PD,Préstamo G

更新日期：1999-03-01 00:00:00

abstract：:Traditional bread (khamir) was made from sorghum flour of two local varieties, Bayadh and Hamra. The bread was prepared by mixing the sorghum flour with water and spices (onion, garlic, lemon juice and fenugreek) in a 1:0.8 (w/w) ratio and fermented for 24 h at 30 degrees C. Two other fermentations were carried out us...

journal_title：Journal of applied microbiology

authors： Gassem MA

更新日期：1999-02-01 00:00:00

abstract：:The effects of nisin and monolaurin, alone and in combination, were investigated on Bacillus licheniformis spores in milk at 37 degrees C. In the absence of inhibitors, germinated spores developed into growing vegetative cells and started sporulation at the end of the exponential phase. In the presence of nisin (25 IU...

journal_title：Journal of applied microbiology

authors： Mansour M,Amri D,Bouttefroy A,Linder M,Milliere JB

更新日期：1999-02-01 00:00:00

abstract：:In order to extend the knowledge on the possible effect of diet on the gastrointestinal microbial community of fish, Arctic charr (Salvelinus alpinus L.) were fed diets containing high (23.7%) and low (6.4%) levels of carbohydrate. The number of viable aerobic and facultative aerobic bacteria associated with the diges...

journal_title：Journal of applied microbiology

authors： Ringø E,Olsen RE

更新日期：1999-01-01 00:00:00